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Corning Life Sciences microarray hybridization chambers
Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
Microarray Hybridization Chambers, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridization+chamber/microarray+hybridization+chambers/pmc03733744-47-7-10
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microarray hybridization chambers - by Bioz Stars, 2026-09
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1) Product Images from "Overexpression of primary microRNA 221/222 in acute myeloid leukemia"

Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

Journal: BMC Cancer

doi: 10.1186/1471-2407-13-364

Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
Figure Legend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

Techniques Used: Microarray, Quantitative RT-PCR, Expressing

Related Articles

Hybridization:

Article Title: Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis
Article Snippet: .. Hybridization was in a Corning Microarray Hybridization Chamber (Corning Inc.) in 42°C water bath. .. Microarray slides were scanned with a Virtek ChipReader (Virtek Vision, Waterloo, ON, Canada).

Article Title: Proteomic Monitoring of B Cell Immunity
Article Snippet: HybriWell gasket adhesive (Grace Bio-Labs, Bend, OR, USA). .. Microarray Hybridization Chamber (Corning, Corning, NY, USA). ..

Article Title: The CLIP-CHIP oligonucleotide microarray: dedicated array for analysis of all protease, nonproteolytic homolog, and inhibitor gene transcripts in human and mouse.
Article Snippet: The CLIP-CHIP oligonucleotide microarray allows the analysis of mRNA transcript levels in a tissue sample for all proteases, nonproteolytic homologs, and protease inhibitors of the human and mouse genome.. In the protocol presented in this unit, total RNA is extracted from a tissue, and the resulting mRNA is reverse transcribed into cDNA and dsDNA and then amplified in an in vitro transcription reaction.. The amplified antisense RNA is labeled with a fluorescent dye and hybridized to the CLIP-CHIP, which contains unique oligonucleotides that are specifically designed for the protease, nonproteolytic homologs, protease inhibitors, and control samples.

Article Title: Sensitivity of 70-mer oligonucleotides and cDNAs for microarray analysis of gene expression in Arabidopsis and its related species
Article Snippet: .. After covering the array with a 24 × 40 mm coverslip (Sigma, St Louis, MO), the slide was placed in a microarray hybridization chamber (Corning Incorporated, Corning, NY). ..

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..

Article Title: Rhb1 Regulates the Expression of Secreted Aspartic Protease 2 through the TOR Signaling Pathway in Candida albicans
Article Snippet: .. Slides were prehybridized with 1% BSA, washed, and hybridized with Cy3- and Cy5-labeled aRNA in a Corning microarray hybridization chamber (catalog no. 2551; Corning). ..

Article Title: Gene Expression Profiling Identifies Lobe-Specific and Common Disruptions of Multiple Gene Networks in Testosterone-Supported, 17?-Estradiol- or Diethylstilbestrol-Induced Prostate Dysplasia in Noble Rats
Article Snippet: Then Cy3 fluorescent dye was coupled to the cDNAs derived from individual prostatic lobes, whereas Cy5 dye was conjugated to the universal rat reference RNA obtained from Stratagene (La Jolla, CA). .. Equal quantities of two labeled probes were mixed and hybridized in a Corning microarray hybridization chamber (Corning, Corning, NY) at 50°C overnight (≥16 hours). ..

Article Title: Separation of the bacterial species, Escherichia coli, from mixed-species microbial communities for transcriptome analysis.
Article Snippet: .. Hybridization was in a Corning Microarray Hybridization Chamber (Corning Inc.) in 42°C water bath. .. Microarray slides were scanned with a Virtek ChipReader (Virtek Vision, Waterloo, ON, Canada).

Microarray:

Article Title: Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis
Article Snippet: .. Hybridization was in a Corning Microarray Hybridization Chamber (Corning Inc.) in 42°C water bath. .. Microarray slides were scanned with a Virtek ChipReader (Virtek Vision, Waterloo, ON, Canada).

Article Title: Proteomic Monitoring of B Cell Immunity
Article Snippet: HybriWell gasket adhesive (Grace Bio-Labs, Bend, OR, USA). .. Microarray Hybridization Chamber (Corning, Corning, NY, USA). ..

Article Title: The CLIP-CHIP oligonucleotide microarray: dedicated array for analysis of all protease, nonproteolytic homolog, and inhibitor gene transcripts in human and mouse.
Article Snippet: The CLIP-CHIP oligonucleotide microarray allows the analysis of mRNA transcript levels in a tissue sample for all proteases, nonproteolytic homologs, and protease inhibitors of the human and mouse genome.. In the protocol presented in this unit, total RNA is extracted from a tissue, and the resulting mRNA is reverse transcribed into cDNA and dsDNA and then amplified in an in vitro transcription reaction.. The amplified antisense RNA is labeled with a fluorescent dye and hybridized to the CLIP-CHIP, which contains unique oligonucleotides that are specifically designed for the protease, nonproteolytic homologs, protease inhibitors, and control samples.

Article Title: Sensitivity of 70-mer oligonucleotides and cDNAs for microarray analysis of gene expression in Arabidopsis and its related species
Article Snippet: .. After covering the array with a 24 × 40 mm coverslip (Sigma, St Louis, MO), the slide was placed in a microarray hybridization chamber (Corning Incorporated, Corning, NY). ..

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..

Article Title: Rhb1 Regulates the Expression of Secreted Aspartic Protease 2 through the TOR Signaling Pathway in Candida albicans
Article Snippet: .. Slides were prehybridized with 1% BSA, washed, and hybridized with Cy3- and Cy5-labeled aRNA in a Corning microarray hybridization chamber (catalog no. 2551; Corning). ..

Article Title: Gene Expression Profiling Identifies Lobe-Specific and Common Disruptions of Multiple Gene Networks in Testosterone-Supported, 17?-Estradiol- or Diethylstilbestrol-Induced Prostate Dysplasia in Noble Rats
Article Snippet: Then Cy3 fluorescent dye was coupled to the cDNAs derived from individual prostatic lobes, whereas Cy5 dye was conjugated to the universal rat reference RNA obtained from Stratagene (La Jolla, CA). .. Equal quantities of two labeled probes were mixed and hybridized in a Corning microarray hybridization chamber (Corning, Corning, NY) at 50°C overnight (≥16 hours). ..

Article Title: Separation of the bacterial species, Escherichia coli, from mixed-species microbial communities for transcriptome analysis.
Article Snippet: .. Hybridization was in a Corning Microarray Hybridization Chamber (Corning Inc.) in 42°C water bath. .. Microarray slides were scanned with a Virtek ChipReader (Virtek Vision, Waterloo, ON, Canada).

Incubation:

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..

Labeling:

Article Title: Gene Expression Profiling Identifies Lobe-Specific and Common Disruptions of Multiple Gene Networks in Testosterone-Supported, 17?-Estradiol- or Diethylstilbestrol-Induced Prostate Dysplasia in Noble Rats
Article Snippet: Then Cy3 fluorescent dye was coupled to the cDNAs derived from individual prostatic lobes, whereas Cy5 dye was conjugated to the universal rat reference RNA obtained from Stratagene (La Jolla, CA). .. Equal quantities of two labeled probes were mixed and hybridized in a Corning microarray hybridization chamber (Corning, Corning, NY) at 50°C overnight (≥16 hours). ..



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Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide <t>microarray</t> data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.
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Image Search Results


Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

Journal: BMC Cancer

Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

doi: 10.1186/1471-2407-13-364

Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

Techniques: Microarray, Quantitative RT-PCR, Expressing

A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

Journal: PLoS ONE

Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression

doi: 10.1371/journal.pone.0110934

Figure Lengend Snippet: A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

Article Snippet: Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to Agilent's one-color microarray-based gene expression analysis protocol (Version 5.7, March 2008).

Techniques: Expressing, Microarray, Derivative Assay